Three months after the events of the fourth film, Manny and his crew get ready for Easter. With Ellie struggles to make the decorations single-handedly, Manny and Diego try to watch some eagle fights between and an eagle and different animals, Crash and Eddie try repeated unsuccessful attempts to prank Peaches (starting the first April Fools Day), who wants to spend Easter with her friends, and Scrat once again loses his acorn when it becomes a tree. In the meantime, Sid decides to look after eggs for the owners of them, much to the scorn of Manny and Diego.
Meanwhile, ex-pirate bunny Squint has been living in his home hole for the past three months with his lazy brother, Clint. After getting back on his feet, the pirate sees Manny and his group as he plans to have his revenge for what he did to Captain Gutt and his boat. Squint confronts them, demanding a new boat, but is unsuccessful. However, he steals the eggs when Sid falls asleep, forcing the sloth and his friends to look for them and Clint lands a hand by giving them a map to the eggs, which have been painted by Squint to camouflage them. When they retrieve the eggs and see through their disguise, they find they are one egg short, with Squint telling them that if they don’t make a boat for him by the next day, the egg will be scrambled.
Clint leads his brother into an ambush by Manny, Sid, and Diego. They find the egg when it’s revealed to be painted into an acorn and has been taken by Scrat. When Manny washes the paint off, a disappointed Scrat throws the egg off a cliff and the gang manage to retrieve it. Squint battles with Sid to get the egg, but the ice cracks and Squint is eventually led into Crash and Eddie’s latest prank. After that, the mammoth family decide to spend Easter together and Sid suggests the Clint be the Easter Bunny by painting eggs every year and hiding them. Meanwhile, a defeated Squint uses Scrat’s nut basket as a ship, but it sinks and Scrat is unable to stop him due to being covered in chocolate that dries up like cement.